Introduction to Biosafety Cabinets

  • Course Price: $150 USD
  • BIO Member Price: $120 USD
  • Course Run Time: 44 minutes
  • Course Level: Level 1 - Foundational
  • Completion Period: 2 week

Introduction to Biosafety Cabinets provides an overview of biosafety cabinets (BSCs), including the different types of cabinets, safe operation and work practices, proper decontamination methods, and a review of the NSF/ANSI 49 Standard.

  1. Function and use of various biosafety cabinets and HEPA filters.
  2. List of what class II and III biosafety cabinets (BSC) protect.
  3. Understanding of the BSC certification process and the differnet functional tests they must undergo.
  4. Fluency in the primary method of decontamination and types of decontaminates approved by ANSI.
  5. Download of NSF/ANFI 49 Standard.
Section 1: Air Filtration
Explains the features and functions of a BSC and HEPA filter, and lists the differences in retaining particle sizes for HEPA, ULPA, and SULPA filters.
Section 2: Types of Biosafety Cabinets
Describes types of BSCs, their differences, and what each is used for.
Section 3: Working in the Biosafety Cabinet
Defines what class II and class III cabinets protect and explains why UV lights are not recommended for use in a BSC.
Section 4: NSF/ANSI 49-2009
Explains BSCs certification and describes the different functional tests for BSCs.
Section 5: Biosafety Cabinet Decontamination
Describes the primary method of decontamination, lists the types of decontaminants approved by ANSI, and explains what indicators are used to validate decontamination.
Final Quiz
Course Evaluation
You have two weeks from time of purchase to complete any of the online courses. For example, if you purchase the course at 9 am on Saturday, the course will close at 9 am 14 days later.

Our online classes are between 40 minutes and 85 minutes in length. We suggest allowing 20 minutes to take the end of the course quiz and 10 minutes to complete the class evaluation. 

There are no refunds once you have registered for an online course. 

The online classes are fully animated with a professional voice-over. Each course begin with an overview of what you will learn, the course itself, and then ends with a course quiz to assess your understanding.

You can repeat the class as often as you like within your two week run time.

You have two chances to pass the quiz. A passing score is 70% or higher. 

Each course is to be taken by one individual. If you would like to take our online courses as a group, please contact Stacey Hawkins at This email address is being protected from spambots. You need JavaScript enabled to view it. for pricing.

There are three requirements to receive your course certificate.

  1. You must view the entire course.
  2. You must pass the course quiz with a 70%.
  3. You must complete the course evaluation.

You can download and print your certificate from your dashboard only when all three of these items are completed. 

Before moving on to the next section of our on-demand, online courses, all slides within a section must be viewed.

There is an easy way for you to see which slides you have completed and not completed in the course. Look at the menu on the left side of the course- the slides that have been seen are darkened and the slides that have not been seen are light. 


To access the BIO member discount, use your company email address during the checkout process. The checkout system will automatically recognize your organization and apply the 20% discount to your purchase.  

If your course has expired, but you have not completed it, you can extend it for an additional 2 weeks for $25.

  • Log into your account
  • Go to the course page you would like to extend
  • Click on the "course extension" button. This will bring you to the payment gateway.
  • Please note: It will appear you are being asked to pay the full amount of $150, but a discount will be automatically given once you fill in all of your payment information. 

You have up until 90 days from the day your course expired to take advantage of this discounted fee. Once the 90 days are past, you will need to pay the full price to view the course again. 

  1. When you launch your online course (any section) look at the upper right-hand corner of the screen and click on “Resources”.
  2. A drop down box will appear that says “Course Slides”
  3. Click on "Course Slides". You can then download and/or print the slides.