How Diagnostic Tests Work: Protein-Based Diagnostics

  • Course Price: $150 USD
  • BIO Member Price: $120 USD
  • Course Run Time: 40 minutes
  • Course Level: Level 2 - General
  • Completion Period: 2 week

How Diagnostics Work: Protein-Based Diagnostics focuses on antibodies: what they are, where they come from and how they work. Various antibody-based diagnostics, such as sandwich and bead immunoassays, multiplexed assays, lateral flow assays and chromatography, are explained in detail. Develop an understanding of these diverse tools and how to interpret results, knowledge which can be applied in research, drug development, and patient care.

Suggested Prerequisites

Biology of Biotech
Diagnostics’ Role in Medicine Today
Statistical Features of Diagnostics

  1. Define the various types of protein-based diagnostics.
  2. An improved ability to explain how biomarkers are used in diagnostics.
  3. Explain how antibodies are used in diagnostics.
  4. Understand the fundamental science of protein-based diagnostics.
  5. Interpret the information gained from the following assays: antibody diagnostics, ELISA, bead immunoassays, lateral flow assays and chromatography diagnostics.
Certificate Requirements
  • View the entire course
  • Pass course exam with a 70 percent
  • Complete the course evaluation
Section 1: Defining Protein-Based Diagnostics
Introduces protein-based diagnostics by explaining the importance of biomarkers in diagnostics. A biomarker is a measurable molecule indicative of disease.
Section 2: Antibody Technology
Explains the importance of antibody structure and how that structure contributes to its function. Antibody structure and function are exploited by the...More
Section 3: Enzyme-Linked Immunosorbent Assays (ELISA) Technology
Demonstrates the technology and how to interpret results of the widely-used screening test known as an ELISA.
Section 4: Bead Immunoassay Technology
Extends your ELISA knowledge by showing how ELISA technology can be adapted into more high throughput techniques, such as multiplexed bead assays.
Section 5: Lateral Flow Assay Technology
Explains how lateral flow immunochromatographic assay works, its applications and how to interpret its results.
Section 6: Chromatography Technology
Describes the applications of column chromatography in diagnostics and how to interpret its results.
Final Quiz
Course Evaluation
Certificate Requirements
  • View the entire course
  • Pass course exam with a 70 percent
  • Complete the course evaluation
You have two weeks from time of purchase to complete any of the online courses. For example, if you purchase the course at 9 am on Saturday, the course will close at 9 am 14 days later.

Our online classes are between 40 minutes and 85 minutes in length. We suggest allowing 20 minutes to take the end of the course quiz and 10 minutes to complete the class evaluation. 

There are no refunds once you have registered for an online course. 

The online classes are fully animated with a professional voice-over. Each course begin with an overview of what you will learn, the course itself, and then ends with a course quiz to assess your understanding.

You can repeat the class as often as you like within your two week run time.

You have two chances to pass the quiz. A passing score is 70% or higher. 

Each course is to be taken by one individual. If you would like to take our online courses as a group, please contact Stacey Hawkins at This email address is being protected from spambots. You need JavaScript enabled to view it. for pricing.

There are three requirements to receive your course certificate.

  1. You must view the entire course.
  2. You must pass the course quiz with a 70%.
  3. You must complete the course evaluation.

You can download and print your certificate from your dashboard only when all three of these items are completed. 

Before moving on to the next section of our on-demand, online courses, all slides within a section must be viewed.

There is an easy way for you to see which slides you have completed and not completed in the course. Look at the menu on the left side of the course- the slides that have been seen are darkened and the slides that have not been seen are light. 


To access the BIO member discount, use your company email address during the checkout process. The checkout system will automatically recognize your organization and apply the 20% discount to your purchase.  

If your course has expired, but you have not completed it, you can extend it for an additional 2 weeks for $25.

  • Log into your account
  • Go to the course page you would like to extend
  • Click on the "course extension" button. This will bring you to the payment gateway.
  • Please note: It will appear you are being asked to pay the full amount of $150, but a discount will be automatically given once you fill in all of your payment information. 

You have up until 90 days from the day your course expired to take advantage of this discounted fee. Once the 90 days are past, you will need to pay the full price to view the course again. 

  1. When you launch your online course (any section) look at the upper right-hand corner of the screen and click on “Resources”.
  2. A drop down box will appear that says “Course Slides”
  3. Click on "Course Slides". You can then download and/or print the slides.
Certificate Requirements
  • View the entire course
  • Pass course exam with a 70 percent
  • Complete the course evaluation